1. MSU用PBS重悬， 每只实验鼠腹腔注射1mgMSU;
2. 6小时后取腹腔灌洗液， 用10mL PBS冲洗；
3. 分析灌洗液中中性粒细胞数量，流式细胞检测，细胞表面分子： Ly-6G和CD11b
Brief protocol of MSU induced mouse peritonitis model
1. Mice were i.p. injected of 1mg MSU crystals in 0.5 ml PBS;
2. 6h later, animals were killed by CO2 exposure;
3. Peritoneal cavities were washed with 10 ml of PBS;
4. The lavage fluids were analyzed for neutrophil recruitment by fluorescence-activated cell sorting using the neutrophil markers Ly-6G and CD11b
